Journal: Stem Cells Translational Medicine
Article Title: Autophagy modulates tenogenic differentiation of cartilage-derived stem cells in response to mechanical tension via FGF signaling
doi: 10.1093/stcltm/szae085
Figure Lengend Snippet: Autophagy influences the tenogenic differentiation of cyclic tensile stress-treated CDSCs. (A) Flow cytometry analysis was performed to evaluate the expression of mouse mesenchymal stem cell-related markers CD90.2, CD44, CD29, and CD34 in P3 passage mouse CDSCs. (B) Time-lapse images show the morphological changes of CDSCs under cyclic tensile stress (5%, 1HZ). (C-D) PCR (C) and Western Blot (D) analyses were conducted to assess the expression levels of tenogenic and chondrogenic-related markers in CDSCs treated with cyclic tensile stress at different time points ( n = 3). T represents cyclic tension stress. (E) Western blot analysis was performed to examine autophagy-related marker expression in CDSCs treated with cyclic tensile stress at different time points. (F) Time-lapse images demonstrate the cellular morphology changes of CDSCs under both cyclic tensile stress and chloroquine treatment (40 μmol/L). (G-H) PCR (G) and Western Blot (H) analyses were carried out to determine the expression levels of tenogenic and chondrogenic-related markers in CDSCs subjected to both cyclic tensile stress and chloroquine treatment at different time points( n = 3). CQ denotes chloroquine. (I) Western blot analysis was conducted to investigate autophagy-related marker expression in CDSCs treated with cyclic tensile stress and chloroquine at different time points. Data are mean ± SD. *, P < .05; **, P < .01; ***, P < .001; ****, P < .0001.
Article Snippet: The characteristics of CDSCs were validated using the Mesenchymal Stem Cell (Mouse) Surface Marker Identification Kit (MUXMX-09011, Cyagen).
Techniques: Flow Cytometry, Expressing, Western Blot, Marker